The kit assay Chicken T COL level in the Samples, use Purified Chicken T COL antibody to coat microtiter plate wells, make solid-phase antibody, then add Samples (Containing Chicken T COL) to wells, combined Chicken T COL antidody which with HRP labeled, become antibody – antigen – enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change (yellow) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Chicken T COL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Ordering Information: Cat#GEN-TCOL – Size: 96T