BlueFeather, the singleton that wasn't: Shared gene content analysis supports expansion of Arthrobacter phage Cluster FE

BlueFeather, the singleton that wasn’t: Shared gene content analysis supports expansion of Arthrobacter phage Cluster FE

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Bacteriophages (phages) exhibit excessive genetic variety, and the mosaic nature of the shared genetic pool makes quantifying phage relatedness a shifting goal. Early parameters for clustering of associated Mycobacteria and Arthrobacter phage genomes relied on nucleotide identification thresholds however, extra just lately, clustering of Gordonia and Microbacterium phages has been carried out in line with shared gene content. Singleton phages lack the nucleotide identification and/or shared gene content required for clustering newly sequenced genomes with identified phages.
Whole genome metrics of novel Arthrobacter phage BlueFeather, initially designated a putative singleton, confirmed low nucleotide identification however excessive amino acid and gene content similarity with Arthrobacter phages initially assigned to Clusters FE and FI. Gene content similarity revealed that BlueFeather shared genes with these phages in extra of the parameter for clustering Gordonia and Microbacterium phages. Single gene analyses revealed proof of horizontal gene switch between BlueFeather and phages in distinctive clusters that infect a spread of bacterial hosts. Our findings spotlight the benefit of utilizing shared gene content to check seemingly genetically remoted phages and have resulted in the reclustering of BlueFeather, a putative singleton, in addition to former Cluster FI phages, right into a newly expanded Cluster FE

Isolation and Characterization of Shiga Toxin Bacteriophages

Shiga toxin (Stx) phages might be induced from Stx-producing Escherichia coli strains (STEC) or might be remoted as free virions from completely different samples. Here we describe strategies used for the detection, enumeration, and isolation of Stx bacteriophages. Stx phages are temperate phages positioned in the genome of STEC. Their induction from the host pressure cultures is achieved by completely different inducing brokers, mitomycin C being one of the mostly used.
Detection of infectious Stx phages requires the manufacturing of seen plaques in a confluent garden of the host pressure utilizing a double agar layer technique. However, as the plaques produced by Stx phages are sometimes barely seen and there’s a risk that non-Stx phages may also be induced from the pressure, a hybridization step ought to be added to acknowledge and correctly enumerate the lysis plaques generated after induction. Molecular strategies may also be used to determine and enumerate Stx phages.
Real-time quantitative PCR (qPCR) is the most correct technique for absolute quantification, though it can not decide the infectivity of Stx phages. qPCR may also be helpful for the detection of free Stx phage virions in numerous pattern sorts.Stx phages induced from lysogenic bacterial strains might be purified by cesium chloride density gradients; this protocol additionally helps to particularly discriminate Stx phages from different prophages current in the genome of the host pressure by choosing the phages expressing the Stx gene. High titer suspensions of Stx phages obtained after induction of giant volumes of bacterial cultures and lysate focus permits phage characterization by electron microscopy research and genomic analysis.

Expression of virulence elements by Pseudomonas aeruginosa biofilm after bacteriophage an infection

The use of bacteriophages for the therapy of bacterial infections has been extensively studied. Nonetheless, the stress response concerning bacteriophage an infection and the expression of virulence elements of Pseudomonas aeruginosa after phage an infection is poorly mentioned. In this examine, we evaluated biofilm formation capability and expression of virulence elements of P. aeruginosa after bacteriophage an infection.
Biofilm progress charges, biofilm morphology, pyocyanin manufacturing and elastase exercise have been evaluated after 2, 8, 24 and 48 h of co-cultivation with bacteriophages that was just lately characterised and confirmed to be infective in direction of medical isolates.
BlueFeather, the singleton that wasn't: Shared gene content analysis supports expansion of Arthrobacter phage Cluster FE
In parallel, quantitative real-time polymerase chain reactions have been carried out to confirm the expression of virulence-related genes. Bacteriophages promoted substantial modifications in P. aeruginosa biofilm progress at early co-culture time. In addition, at Eight h, we noticed that some cultures developed filaments. Although bacteriophages didn’t alter each pyocyanin and protease exercise, modifications on the expression stage of genes associated to virulence elements have been detected. Usually, lasI, pslA, lasB and phzH genes have been upregulated after 2 and 48 h of co-culture. These outcomes spotlight the want for in depth investigation of pathways and molecules concerned in phage an infection, since the transcriptional modifications would recommend a response activation by P. aeruginosa.

Structural Insights into gp16 ATPase in the Bacteriophage ϕ29 DNA Packaging Motor

Biological motors, ubiquitous in dwelling methods, convert chemical vitality into completely different sorts of mechanical motions crucial to mobile features. Gene product 16 (gp16) in bacteriophage ϕ29 is amongst the strongest biomotors identified, which adopts a multisubunit ring-shaped construction and hydrolyzes ATP to package deal double-stranded DNA (dsDNA) right into a preformed procapsid. Here we report the crystal construction of the C-terminal area of gp16 (gp16-CTD).
Structure-based alignment and molecular dynamics simulations revealed a necessary binding floor of gp16-CTD for prohead RNA, a singular part of the motor complicated. Furthermore, our simulations highlighted a dynamic interaction between the N-terminal area and the CTD of gp16, which can play a task in driving motion of DNA into the procapsid. Lastly, we assembled an atomic structural mannequin of the full ϕ29 dsDNA packaging motor complicated by integrating structural and experimental information from a number of sources. Collectively, our findings supplied a refined inchworm-revolution mannequin for dsDNA translocation in bacteriophage ϕ29 and prompt how the particular person domains of gp16 work collectively to energy such translocation.

Mapping the purposeful panorama of the receptor binding area of T7 bacteriophage by deep mutational scanning

The interplay between a bacteriophage and its host is mediated by the phage’s receptor binding protein (RBP). Despite its basic function in governing phage exercise and host vary, molecular guidelines of RBP perform stay a thriller. Here, we systematically dissect the purposeful function of each residue in the tip area of T7 phage RBP (1660 variants) by growing a high-throughput, locus-specific, phage engineering technique.
This wealthy dataset allowed us to cross examine purposeful profiles throughout hosts to exactly determine areas of purposeful significance, many which have been beforehand unknown. Substitution patterns confirmed host-specific variations in place and physicochemical properties of mutations, revealing molecular adaptation to particular person hosts.

P1
5-01675 4 x 1mg Ask for price

Cre Recombinant Adenovirus
ADV-005 50 ?L
EUR 891
Description: Premade recombinant adenovirus containing the Cre gene

Cre Rabbit pAb
A18151-100ul 100 ul
EUR 308

Cre Rabbit pAb
A18151-200ul 200 ul
EUR 459

Cre Rabbit pAb
A18151-20ul 20 ul
EUR 183

Cre Rabbit pAb
A18151-50ul 50 ul
EUR 223

Anti-Cre antibody
STJ11100121 100 µl
EUR 277

Anti-Cre antibody
STJ140003 300 µg
EUR 270
Description: Goat polyclonal antibody to Cre recombinase. Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme (38 kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites (loxP sites).

pBS185 CMV-Cre
PVT12294 2 ug
EUR 703

pBS513 EF1alpha-cre
PVT19014 2 ug
EUR 300

pCP- Cre Plasmid
PVT6101 2 ug
EUR 266

pCGlobin2- Cre Plasmid
PVT6102 2 ug
EUR 266

Luciferase-2A-CRE, (GFP-Bsd) lentiviral particles
LVP411 1x107 IFU/ml x 200ul
EUR 507
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A GFP-Blasticidin fusion dual marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

Luciferase-2A-CRE, (GFP-Puro) lentiviral particles
LVP412 1x107 IFU/ml x 200ul
EUR 507
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A GFP-Puromycin fusion dual marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

Luciferase-2A-CRE, (RFP-Bsd) lentiviral particles
LVP413 1x107 IFU/ml x 200ul
EUR 507
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A RFP-Blasticidin fusion dual marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

Luciferase-2A-CRE, (RFP-Puro) lentiviral particles
LVP414 1x107 IFU/ml x 200ul
EUR 507
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A RFP-Puromycin fusion dual marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

Recombinant (E. Coli) Creatininase (500 U/mg)
CRE-02 500,000 U
EUR 2760

M13 Bacteriophage Antibody (Biotin)
abx413451-01mg 0.1 mg
EUR 1010

P1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV739457 1.0 ug DNA Ask for price

P1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV739461 1.0 ug DNA Ask for price

P1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV739462 1.0 ug DNA Ask for price

Micrococcin P1
M055-0.5MG 0.5 mg
EUR 318

Micrococcin P1
M055-2.5MG 2.5 mg
EUR 1040

BTM - P1
5-00870 4 x 1mg Ask for price

P1 Protein
abx160013-1mg 1 mg
EUR 1678

BTM-P1
SP-88321-1 1 mg
EUR 225

Cecropin P1
H-5718.0500 0.5mg
EUR 139
Description: Sum Formula: C147H253N45O43; CAS# [125667-96-1]

Cecropin P1
H-5718.1000 1.0mg
EUR 206
Description: Sum Formula: C147H253N45O43; CAS# [125667-96-1]

CRE (Neo), CMV lentivirus
LVP297 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase with Neomycin marker, provided in DMEM medium with 10% FBS.

CRE (Bsd), CMV lentivirus
LVP336 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase with Blasticidin marker, provided in DMEM medium with 10% FBS.

CRE (Puro), CMV lentivirus
LVP339 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase with Puromycin marker, provided in DMEM medium with 10% FBS.

CRE (Bsd), EF1a lentivirus
LVP519 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with Blasticidin marker, provided in DMEM medium with 10% FBS.

CRE (Puro), EF1a lentivirus
LVP520 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with Puromycin marker, provided in DMEM medium with 10% FBS.

CRE (Neo), EF1a lentivirus
LVP521 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with Neomycin marker, provided in DMEM medium with 10% FBS.

CRE (Puro), CAG lentivirus
LVP573 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under CAG promoter with Puromycin marker, provided in DMEM medium with 10% FBS.

CRE (Bsd), CAG lentivirus
LVP574 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under CAG promoter with Blasticidin marker, provided in DMEM medium with 10% FBS.

CRE (Neo), CAG lentivirus
LVP575 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under CAG promoter with Neomycin marker, provided in DMEM medium with 10% FBS.

AAV2-Cre Control Virus
AAV-310 50 ?L
EUR 1018
Description: Cre control virus of AAV serotype 2.

AAV1-Cre Control Virus
AAV-311 50 ?L
EUR 1018
Description: Cre control virus of AAV serotype 1.

AAV3-Cre Control Virus
AAV-313 50 ?L
EUR 1018
Description: Cre control virus of AAV serotype 3.

AAV4-Cre Control Virus
AAV-314 50 ?L
EUR 1018
Description: Cre control virus of AAV serotype 4.

AAV5-Cre Control Virus
AAV-315 50 ?L
EUR 1018
Description: Cre control virus of AAV serotype 5.

AAV6-Cre Control Virus
AAV-316 50 ?L
EUR 1018
Description: Cre control virus of AAV serotype 6.

Cre-IRES-PuroR Plasmid
PVT17083 2 ug
EUR 325

pGL4.29[luc2P- CRE- Hygro]
PVT10799 2 ug
EUR 301

Der-P1 Der P1 Protein Recombinant Protein
PROTP08176 Regular: 0.5mg
EUR 788
Description: The E.Coli derived recombinant protein contains the Dermatophagoides pteronyssinus Dust Mite Der P1 protein (a.a. 20-320) and fused to a 6 His Tag at C-terminus, having a total Mw of 34.5kDa, pI 5.6.

P1 ORF Vector (Human) (pORF)
ORF027336 1.0 ug DNA Ask for price

Luciferase-2A-CRE (Bsd), lentiviral particles, in vivo ready
LVP304-PBS 5 x107 IFU/ml x 200ul
EUR 862
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A Blasticidin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

Luciferase-2A-CRE (Puro), lentiviral particles, in vivo ready
LVP409-PBS 5 x107 IFU/ml x 200ul
EUR 862
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A Puromycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

Luciferase-2A-CRE (Neo), lentiviral particles, in vivo ready
LVP410-PBS 5 x107 IFU/ml x 200ul
EUR 862
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A Neomycin marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

Cecropin P1 porcine
C214-1MG 1 mg
EUR 274

SZL P1-41
HY-100237 100mg
EUR 1345

Cecropin P1 (porcine)
5-00957 4 x 1mg Ask for price

Der P1 Protein
20-abx260104
  • EUR 885.00
  • EUR 342.00
  • EUR 1372.00
  • 0.5 mg
  • 100 ug
  • 1 mg

Nuclease P1 Antibody
20-abx300888
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

SZL P1-41
B7745-10 10 mg
EUR 321

SZL P1-41
B7745-100 100 mg
EUR 1750

SZL P1-41
B7745-25 25 mg
EUR 628

SZL P1-41
B7745-5 5 mg
EUR 203

SZL P1-41
B7745-5.1 10 mM (in 1mL DMSO)
EUR 218

SZL P1-41
B7745-50 50 mg
EUR 1044

Nuclease P1 Antibody
1-CSB-PA328459LA01PEU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Nuclease P1. Recognizes Nuclease P1 from Penicillium citrinum. This antibody is Unconjugated. Tested in the following application: ELISA

CRE (GFP-Puro), CAG lentivirus
LVP576 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express (under CAG promoter) nuclear permeable CRE recombinase and the GFP marker under the same suCAG promoter. A Puromycin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

CRE (RFP-Bsd), CAG lentivirus
LVP577 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express (under CAG promoter) nuclear permeable CRE recombinase and the RFP marker under the same suCAG promoter. A Blasticidin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

CRE (RFP-Puro), CAG lentivirus
LVP578 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express (under CAG promoter) nuclear permeable CRE recombinase and the RFP marker under the same suCAG promoter. A Puromycin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

CRE-2A-GFP, CMV lentivirus
LVP804 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. No any antibioic selection marker. Virus provided in DMEM medium with 10% FBS.

CRE-2A-RFP, CMV lentivirus
LVP805 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the RFP marker under the same suCMV promoter. No any antibioic selection marker. Virus provided in DMEM medium with 10% FBS.

pGL4.29[luc2P/ CRE/ Hygro] Plasmid
PVT1312 2 ug
EUR 266

AAV- U6gRNA1- U6gRNA2- TnT- Cre
PVT10895 2 ug
EUR 266

Mouse Anti M13 Bacteriophage Monoclonal Antibody
DMABT-54839MM 0.25 mg
EUR 944

Luciferase-2A-CRE, (GFP-Bsd) lentiviral particles, in vivo ready
LVP411-PBS 5 x107 IFU/ml x 200ul
EUR 862
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A GFP-Blasticidin fusion dual marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

Luciferase-2A-CRE, (GFP-Puro) lentiviral particles, in vivo ready
LVP412-PBS 5 x107 IFU/ml x 200ul
EUR 862
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A GFP-Puromycin fusion dual marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

Luciferase-2A-CRE, (RFP-Bsd) lentiviral particles, in vivo ready
LVP413-PBS 5 x107 IFU/ml x 200ul
EUR 862
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A RFP-Blasticidin fusion dual marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

Luciferase-2A-CRE, (RFP-Puro) lentiviral particles, in vivo ready
LVP414-PBS 5 x107 IFU/ml x 200ul
EUR 862
Description: Pre-made lentiviral particles bicistronically express individual firefly luciferase and nuclear permeable CRE recombinase under the same suCMV promoter. A RFP-Puromycin fusion dual marker was expressed under RSV promoter. Virus concentrated and buffer changed into PBS solution.

Arabidopsis thaliana NADP-dependent alkenal double bond reductase P1 (P1)
1-CSB-EP654400DOA
  • EUR 611.00
  • EUR 309.00
  • EUR 1827.00
  • EUR 939.00
  • EUR 1218.00
  • EUR 397.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arabidopsis thaliana NADP-dependent alkenal double bond reductase P1(P1) expressed in E.coli

Arabidopsis thaliana NADP-dependent alkenal double bond reductase P1 (P1)
1-CSB-EP654400DOAe1
  • EUR 727.00
  • EUR 425.00
  • EUR 1943.00
  • EUR 1055.00
  • EUR 1335.00
  • EUR 513.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Arabidopsis thaliana NADP-dependent alkenal double bond reductase P1(P1) expressed in E.coli

P1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV739458 1.0 ug DNA Ask for price

P1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV739459 1.0 ug DNA Ask for price

P1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV739460 1.0 ug DNA Ask for price

CRE (Neo), CMV lentivirus, in PBS 
LVP297-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase with Neomycin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE (Bsd), CMV lentivirus in PBS 
LVP336-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase with Blasticidin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE-2A-GFP (Bsd), CMV lentivirus
LVP337 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. A Blasticidin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

CRE-2A-RFP (Puro), CMV lentivirus
LVP338 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the RFP marker under the same suCMV promoter. A Puromycin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

CRE (Puro), CMV lentivirus in PBS 
LVP339-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase with puromycin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE-2A-GFP (Puro), CMV lentivirus
LVP407 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. A Puromycin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

CRE-2A-GFP (Neo), CMV lentivirus
LVP408 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express individual nuclear permeable CRE recombinase and the GFP marker under the same suCMV promoter. A Neomycin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

CRE (Bsd), EF1a lentivirus in PBS 
LVP519-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with Blasticidin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE (Puro), EF1a lentivirus in PBS 
LVP520-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with puromycin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE (Neo), EF1a lentivirus, in PBS 
LVP521-PBS 5 x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing nuclear permeable CRE recombinase under EF1a promoter with Neomycin marker. Virus concentrated and buffer changed into provided in PBS solution

CRE-2A-RFP (Bsd), EF1a lentivirus
LVP522 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the RFP marker under the same suEF1a promoter. A Blasticidin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

CRE-2A-RFP (Puro), EF1a lentivirus
LVP523 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the RFP marker under the same suEF1a promoter. A Puromycin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.

CRE-2A-RFP (Neo), EF1a lentivirus
LVP524 1x107 IFU/ml x 200ul
EUR 349
Description: Pre-made lentiviral particles bicistronically express (under EF1a promoter) nuclear permeable CRE recombinase and the RFP marker under the same suEF1a promoter. A Neomycin marker was expressed under RSV promoter. Virus provided in DMEM medium with 10% FBS.
We found gain-of-function variants in opposition to resistant hosts and host-constricting variants that eradicated sure hosts. To reveal therapeutic utility, we engineered extremely lively T7 variants in opposition to urinary tract pathogen. Our method presents a generalized framework for characterizing sequence-function relationships in lots of phage-bacterial methods.